This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Prochownik, E. V. Articles by Kirsten, W. H. Search for Related Content PubMed PubMed Citation Articles by Prochownik, E. V. Articles by Kirsten, W. H.

Induction of Type C Virions from Normal Rat Kidney Cells by 2-Deoxy-D-Glucose

^* Department of Pathology, and Joseph P. Kennedy, Jr., Mental Retardation Research Center, Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637
Department of Pediatrics, and Joseph P. Kennedy, Jr., Mental Retardation Research Center, Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637

ABSTRACT

The sugar 2-deoxy-D-glucose (2-DG) induced the release of type C virions from an established line of normal rat kidney (NRK) cells. Within 20 h after the addition of 5 mg of 2-DG per ml to exponentially growing NRK cultures, more than 80% of the cells expressed the mammalian type C virus interspecies-specific antigen (p30) as determined by indirect cytoplasmic immunofluorescence. Maximal virion release occurred 1 to 2 days after 2-DG was added for 24 h to the growth medium, although a low level of virion production was detected as early as 2.5 h after 2-DG treatment. Studies with inhibitors of RNA synthesis indicated a requirement for de novo RNA synthesis after the addition of 2-DG. Sensitivity of NRK cells to type C virion induction was limited to a relatively short period of in vitro growth and preceded spontaneous virion release by 8 to 10 subculture generations. A model is presented for the sequential derepression of latent type C virus information in serially propagated NRK cells.