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J Virol. 1976 January; 17(1): 212-218
Copyright © 1976 American Society for Microbiology. All Rights Reserved.

Orientation of the Complementary Strands of Polyoma Virus DNA with Respect to the DNA Physical Map

^* Department of Molecular Virology, Imperial Cancer Research Fund Laboratories, London WC2A 3PX, England
M.R.C. Laboratory of Molecular Biology, University Postgraduate Medical School, Cambridge CB2 2QH, England

ABSTRACT

The chemical polarities of the two strands of polyoma virus DNA with respect to the DNA physical map have been determined by hybridization of restriction endonuclease fragments specifically labeled with [¹²⁵I]dCMP at their 3' termini to asymmetric polyoma complementary RNA (the product of in vitro transcription of viral DNA by Escherichia coli RNA polymerase). The orientations of the polyoma-specific stable RNA transcripts present in the cytoplasm of productively infected mouse cells have been deduced from this result: the 5' ends of the early and late viral transcripts map very near the origin of viral DNA replication.

¹ Present address: Department of Radiology, Yale University School of Medicine, New Haven, Conn. 06510.

² Present address: Rockefeller University, New York, N.Y. 10021.