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J Virol. 1976 January; 17(1): 132-139
Copyright © 1976 American Society for Microbiology. All Rights Reserved.

Reactivity of Avian RNA Tumor Viruses with Lectins

¹ Duke University Medical Center, Department of Surgery, Durham, North Carolina 27710

ABSTRACT

The infectivity of avian RNA tumor viruses was inactivated to varying degrees by treatment with either concanavalin A (Con A) or phytohemagglutinin but not by treatment with wheat germ agglutinin. In general, leukosis viruses reacted preferentially with Con A, whereas sarcoma viruses showed more affinity for phytohemagglutinin. In a more extensive study with subgroup A of Prague Rous sarcoma virus (PR-A), the effect of inactivation by Con A could be specifically prevented by the addition of -methyl-D-mannoside, -methyl-D-glucoside, and N-acetyl-D-glucosamine. These sugars were also capable of eluting [³H]glucosamine-labeled material from disrupted PR-A virus, which was bound to a Con A-sepharose affinity column. A major viral glycoprotein recovered from the column had the same mobility as gp85 in polyacrylamide gel electrophoresis and could be immunoprecipitated with anti-gp85 antiserum. These results suggest that the material reacting with Con A is present on the gp85 component of the viral glycoprotein. The diversity in the reactivity of the glycoproteins of transforming and nontransforming viruses with plant lectins is discussed.