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J Virol. 1975 September; 16(3): 741-744

Identification of bacteriophage T4-specific precursor tRNA by using a host mutant defective in the methylation of tRNA.

G R Björk

ABSTRACT

A mutant of Escherichia coli K-12 that is defective in the synthesis of 5-methyluridine (ribothymidine) in tRNA was used to identify precursors to phage T4-specific tRNA. The precursor molecules, isolated by gel filtration, were more than twice the size of tRNA. This method is suitable for isolation of rather large amounts of such precursor molecules.

J Virol. 1975 September; 16(3): 741-744






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Copyright © 1975 by the American Society for Microbiology. All rights reserved.