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J Virol. 1975 September; 16(3): 712-719

Study of the fine structure of adeno-associated virus DNA with bacterial restriction endonucleases.

K I Berns, J Kort, K H Fife, E W Grogan and I Spear

ABSTRACT

A physical map of the adeno-associated virus type 2 genome has been constructed on the basis of the five fragments produced by the restriction endonucleases HindII + III from Hemophilus influenzae. There are three endo R-HindII cleavage sites and one endo R-HindIII site. Evidence has been obtained to support the existence of two nucleotide sequence permutations in adeno-associated virus DNA, the start points of which have been estimated to be separated by 1% of the genome. The three cleavage fragments produced by endo R-Eco RI have been ordered and oriented with respect to the endo R-HindII + III cleavage map.

J Virol. 1975 September; 16(3): 712-719






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J. Bacteriol. Mol. Cell. Biol. Microbiol. Mol. Biol. Rev.
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Copyright © 1975 by the American Society for Microbiology. All rights reserved.