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J Virol. 1975 May; 15(5): 1057-1064
Copyright © 1975 American Society for Microbiology. All Rights Reserved.

Blocked and Unblocked 5' Termini in Reovirus Genome RNA

¹ Department of Cell Biology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110

ABSTRACT

Uniformly ³²P-labeled, double-stranded genome RNA isolated from purified reovirus contains two types of 5'-terminal sequences. One strand contains a phosphatase-resistant 5'-terminal structure, XpppG^*pCpU, which is also present in the viral mRNA. The 5' blocking group, X, is removed by ß-elimination indicating that it is a nucleoside containing free 2',3'-hydroxyls. G^*pC is an alkaline-resistant, 2'-O-methylated sequence. The other strand contains a phosphatase-sensitive 5' sequence, ppGpPupPyp. The results are discussed in relation to blocked 5'-terminal structures in other viral and cellular RNAs.