This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Martin, R G Articles by Chou, J Y Search for Related Content PubMed PubMed Citation Articles by Martin, R G Articles by Chou, J Y

 Previous Article  |  Next Article 

J Virol. 1975 March; 15(3): 599-612

Simian virus 40 functions required for the establishment and maintenance of malignant transformation.

ABSTRACT

Members of the five classes of temperature-sensitive simian virus 40 mutants were tested for their ability to transform Chinese hamster lung cells. Two criteria for transformation were used: the ability to form clones in medium with low serum concentrations and the ability to overgrow a monolayer. Only A mutants failed to transform at the restrictive temperature when subconfluent Chinese hamster lung monolayers were used. However, both A and D mutants failed to transform at the restrictive temperature when confluent monolayers and depleted medium were used. When transformed clones were selected, purified by recloning, and examined at both temperatures, only cell lines induced by A mutants lost the transformed phenotype at the higher temperature. Thus, A function is required for maintenance of the transformed phenotype in Chinese hamster lung cells. A function is known to be required for the initiation of viral DNA synthesis in permissive cells. Therefore, transformation may be a consequence of the introduction into a cell of the capacity for aberrant initiation of DNA replication.

This article has been cited by other articles:

• Javier, R. T., Butel, J. S. (2008). The History of Tumor Virology. Cancer Res. 68: 7693-7706 [Abstract] [Full Text]  
• Gjoerup, O., Zaveri, D., Roberts, T. M. (2001). Induction of p53-Independent Apoptosis by Simian Virus 40 Small t Antigen. J. Virol. 75: 9142-9155 [Abstract] [Full Text]  
• Thompson, W. E., Branch, A., Whittaker, J. A., Lyn, D., Zilberstein, M., Mayo, K. E., Thomas, K. (2001). Characterization of Prohibitin in a Newly Established Rat Ovarian Granulosa Cell Line. Endocrinology 142: 4076-4085 [Abstract] [Full Text]  
• Gjoerup, O., Chao, H., DeCaprio, J. A., Roberts, T. M. (2000). pRB-Dependent, J Domain-Independent Function of Simian Virus 40 Large T Antigen in Override of p53 Growth Suppression. J. Virol. 74: 864-874 [Abstract] [Full Text]  
• Butel, J. S., Lednicky, J. A. (1999). Cell and Molecular Biology of Simian Virus 40: Implications for Human Infections and Disease. JNCI J Natl Cancer Inst 91: 119a-134a [Abstract] [Full Text]  
• Howe, A. K., Gaillard, S., Bennett, J. S., Rundell, K. (1998). Cell Cycle Progression in Monkey Cells Expressing Simian Virus 40 Small t Antigen from Adenovirus Vectors. J. Virol. 72: 9637-9644 [Abstract] [Full Text]  
• Sugino, N., Zilberstein, M., Srivastava, R. K., Telleria, C. M., Nelson, S. E., Risk, M., Chou, J. Y., Gibori, G. (1998). Establishment and Characterization of a Simian Virus 40-Transformed Temperature-Sensitive Rat Luteal Cell Line. Endocrinology 139: 1936-1942 [Abstract] [Full Text]  
• Nathans, D (1979). Restriction endonucleases, simian virus 40, and the new genetics. Science 206: 903-909