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Interactions Between the Vegetative States of Phages and T1

Department of Microbiology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642
Department of Microbiology, The Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina 27103

ABSTRACT

Bacteria containing phage lambda in the vegetative state were produced either by induction of lysogens or by infection of sensitive cells with . These cells were superinfected with T1, and assayed for the production of , T1, or both. Although most of the cells produced only or T1, approximately 10% of the infectious centers were dual yielders. Examination of the progeny phage produced by the population of mixedly-infected cells showed that there was little, if any, phenotypic mixing, as determined by adsorption phenotype. T1am mutants in a variety of T1 genes were tested for their ability to exclude , but none were defective in this ability. One gene of T1, gene 4, can be complemented by .