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J Virol. 1974 December; 14(6): 1337-1342
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Bacillus subtilis DNA Polymerase III Is Required for the Replication of the Virulent Bacteriophage e

Department of Genetics, The Hebrew University of Jerusalem, Jerusalem, Israel

ABSTRACT

The virulent phage e of Bacillus subtilis which contains hydroxymethyluracil in its DNA requires host DNA polymerase III for its DNA replication. DNA polymerase III^ts mutant cells infected with e at restrictive temperatures do not support phage DNA synthesis. However, e grows normally both at low and high temperatures in the mutant's parent strain and in spontaneous DNA polymerase III⁺ revertants isolated from the mutant strain. Temperature-shift-down experiments with e-infected cells having thermosensitive DNA polymerase III (pol III^ts) indicate that at 48 C the thermolabile DNA polymerase III is irreversibly inactivated and has to be synthesized de novo after the shift to 37 C, before phage DNA synthesis can begin. Temperature-shift-up experiments with e-infected mutant cells show that phage replication is arrested immediately after the temperature shift and indicate that e requires DNA polymerase III throughout its replication stage.