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Temperate Bacteriophage Infectious for Asporogenic Variants of Bacillus pumilus

¹ Department of Biological Sciences, University of Maryland Baltimore County, Catonsville, Maryland 21228

ABSTRACT

Bacillus pumilus strain NRRL B-3275 is lysogenic for an inducible, nondefective temperate bacteriophage 75. 75 infects and lysogenizes several asporogenic mutants of B. pumilus strain NRS 576 but does not productively infect the spore⁺ parent. 75 DNA is a linear duplex with a mol wt of about 29 x 10⁶ and a buoyant density of 1.701 g/cm³. The location of the 75 prophage attachment site on the chromosome of both host strains is adjacent to a lysine marker. The apparent order is 75 att lys trp.