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J Virol. 1974 November; 14(5): 1229-1234
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Effects of Actinomycin D on the Cytopathology Induced by Poliovirus in HEp-2 Cells

^a Department of Microbiology, The Ohio State University, Columbus, Ohio 43210

ABSTRACT

One possible mechanism of virus-induced cell damage is that the redistributed (released) lysosomal enzymes produce the cytopathic effect during cytolytic types of infections such as poliovirus in HEp-2 cells. To determine if the lysosomal enzyme redistribution and cell damage are host-cell directed, we studied sensitivity of these events to the action of actinomycin D. By the use of actinomycin D at concentrations producing the least toxicity but maximal effectiveness in shuting down cell RNA synthesis, it was shown that the cytopathic effect and enzyme redistribution were not inhibited and, therefore, not directly controlled and induced by the cell genome in response to the virus infection. Evaluation of cytopathic effect by a phase contrast microscopy method detected changes earlier than the erythrocin B uptake method.

¹ Present address: University of Minnesota, The Hormel Institute, Austin, Minn. 55912