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Reticuloendotheliosis Virus Nucleic Acid Sequences in Cellular DNA

^a McArdle Laboratory, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

Reticuloendotheliosis virus 60S RNA labeled with ¹²⁵I, or reticuloendotheliosis virus complementary DNA labeled with ³H, were hybridized to DNAs from infected chicken and pheasant cells. Most of the sequences of the viral RNA were found in the infected cell DNAs. The reticuloendotheliosis viruses, therefore, replicate through a DNA intermediate. The same labeled nucleic acids were hybridized to DNA of uninfected chicken, pheasant, quail, turkey, and duck. About 10% of the sequences of reticuloendotheliosis virus RNA were present in the DNA of uninfected chicken, pheasant, quail, and turkey. None were detected in DNA of duck. The specificity of the hybridization was shown by competition between unlabeled and ¹²⁵I-labeled viral RNAs and by determination of melting temperatures. In contrast, ¹²⁵I-labeled RNA of Rous-associated virus-O, an avian leukosis-sarcoma virus, hybridized 55% to DNA of uninfected chicken, 20% to DNA of uninfected pheasant, 15% to DNA of uninfected quail, 10% to DNA of uninfected turkey, and less than 1% to DNA of uninfected duck.

¹ Present address: Department of Microbiology, University of Texas, Southwestern Medical School, Dallas, Tex. 75235.

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