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J Virol. 1974 November; 14(5): 1056-1062
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Marker Rescue in Haemophilus influenzae Bacteriophage

^a Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830

ABSTRACT

Rescue of wild-type markers from transfecting phage DNA in cómpetent Haemophilus influenzae cells by superinfection with temperature-sensitive phage (marker rescue) is approximately linearly dependent upon the concentration of transfecting DNA. The amount of marker rescue with a constant amount of transfecting DNA increases with increasing multiplicities of superinfecting phage up to about 4, and then decreases at higher multiplicities. Host restriction of transfecting DNA does not affect marker rescue. The frequency of wild-type recombinants from marker rescue is much greater than that from multiple infection with whole phages, and is comparable to that obtained with two mutant-transfecting DNAs. The amount of marker rescue decreases exponentially with time between entrance of the transfecting DNA and superinfection, and the rate of decrease is independent of map position of the rescued marker. Marker rescue is drastically reduced in the recombination-defective strains, rec1 and rec2.

¹ Present address: Brookhaven National Laboratory, Upton, N.Y. 11973.