This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Carter, B. J. Search for Related Content PubMed PubMed Citation Articles by Carter, B. J.

 Previous Article  |  Next Article 

J Virol. 1974 October; 14(4): 834-839
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Analysis of Parvovirus mRNA by Sedimentation and Electrophoresis in Aqueous and Nonaqueous Solution

¹ Laboratory of Experimental Pathology, National Institute of Arthritis, Metabolism and Digestive Diseases, Bethesda, Maryland 20014
² Laboratory of Biology of Viruses, National Institute of Allergy and Infectious Disease, Bethesda, Maryland 20014

ABSTRACT

Adenovirus-associated virus (AAV)-specific RNA present in the cytoplasm of cells coinfected with a helper adenovirus was analyzed by sucrose gradient sedimentation and gel electrophoresis. In aqueous conditions both gels or gradients revealed three AAV RNA components corresponding to 30S, 27S, and 20S and having apparent molecular weights of 2.6 x 10⁶, 1.75 x 10⁶ to 1.8 x 10⁶, and 0.9 x 10⁶ to 1.0 x 10⁶, respectively. In nonaqueous, denaturing solvents only the 20S AAV RNA species was observed. For this reason, and because they would be apparently significantly larger than a single AAV DNA strand, both the 30S and 27S species are believed to result from conformational or aggregation effects in the aqueous nondenaturing systems. It is concluded that only a single RNA molecule having a molecular weight of approximately 0.9 x 10⁶ to 1.0 x 10⁶ is synthesized by AAV.