This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Neubauer, R. H. Articles by Rabin, H. Search for Related Content PubMed PubMed Citation Articles by Neubauer, R. H. Articles by Rabin, H.

 Previous Article  |  Next Article 

J Virol. 1974 October; 14(4): 745-750
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Stimulation of Herpesvirus saimiri Expression in the Absence of Evidence for Type C Virus Activation in a Marmoset Lymphoid Cell Line

Department of Virology and Cell Biology, Litton Bionetics, Inc., Kensington, Maryland 20795

ABSTRACT

Nucleic acid base analogues were used to examine a Herpesvirus saimiri (HVS)-infected marmoset lymphoid cell line (MLC-1) for possible association with type C viruses. Synthetic templates poly(rA)·d(pT)₁₀ and poly(dA)·d(pT)₁₀ were used to detect RNA-directed DNA polymerase activity in 100-fold concentrated tissue culture fluids. HVS was monitored by immunofluorescence for early, late, and membrane antigens. MLC-1 cells were exposed to 30 µg of 5-bromo-2'-deoxyuridine (BUdR) per ml for 24 h and examined daily. Similar experiments used 5-iodo-2'-deoxyuridine (IUdR) (20 µg/ml) for 30 h or IUdR (20 µg/ml) for 3 days followed by 2% dimethyl sulfoxide for 4 days. Results of these experiments failed to show any type C virus-like polymerase; however, HVS expression was greatly stimulated. BUdR and IUdR enhanced expression of HVS-associated antigens five- to sevenfold, with maximal stimulation being observed 3 to 4 days after removal of the analogue. IUdR-dimethyl sulfoxide treatment was generally less effective. Although more cells showed HVS antigens, the treatments did not increase cell-free infectious virus. The data suggest that HVS-infected lymphoid cells can be stimulated to express virus in a manner similar to that of the Epstein-Barr virus in Burkitt's lymphoma cells. No evidence of type C virus was found in stimulated cultures.