This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Brakel, C. Articles by Kates, J. R. Search for Related Content PubMed PubMed Citation Articles by Brakel, C. Articles by Kates, J. R.

 Previous Article  |  Next Article 

J Virol. 1974 October; 14(4): 724-732
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Poly(A) Polymerase from Vaccinia Virus-Infected Cells II. Product and Primer Characterization

^a Department of Chemistry, University of Colorado, Boulder, Colorado 80302

ABSTRACT

The product of the in vitro reaction of a vaccinia virus-induced poly(A) polymerase (see preceding paper) with ATP is shown to be poly(A) by nuclease resistance and by annealing with poly(U). Polyacrylamide gel electrophoresis indicates that the in vitro synthesized poly(A) is associated with large RNA which is sensitive to RNase. RNA which co-purifies with the virus-induced enzyme is similar to vaccinia virus-specific RNA with respect to size and poly(A) content. Double labeling studies indicate that the RNA which co-purifies with the enzyme becomes associated with the poly(A) synthesized in vitro. The poly(A) formed in vitro is located on the 3'-OH terminus of this RNA. During in vitro poly(A) synthesis ³²P from -[³²P]ATP is transferred to nucleosides other than 2',3'-AMP, primarily to CMP. Inclusion of poly(U) in the in vitro reactions results in an increase in the transfer of ³²P to UMP.

¹ Present Address: University of California at San Francisco, Department of Biochemistry and Biophysics, San Francisco, Calif. 94143.

² Present Address: State University of New York at Stony Brook, Department of Microbiology, Stony Brook, N.Y. 11790.