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J Virol. 1974 October; 14(4): 1017-1021
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Quantitation of Endogenous C-type Virion Production in Several Murine Cell Lines

Ronald B. Luftig, Paul N. Mcmillan and Mollie Gudger

1 Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545

ABSTRACT

Quantitation by enumeration of virion particles, measurement of absorbancy at 260 nm, and densitometry on sodium dodecyl sulfate-polyacrylamide gels has shown that mouse L-M cells yielded 7- to 10-fold less endogenous C-type virions than the parental lines, L or L929. The previously noted stimulation of L-M cell virion production by a concomitant 10% increase in fetal calf serum concentration (D. A. Kindig, R. Karp, and W. H. Kirsten, 1968), was not observed.

J Virol. 1974 October; 14(4): 1017-1021
Copyright © 1974 American Society for Microbiology. All Rights Reserved.





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Copyright © 1974 by the American Society for Microbiology. All rights reserved.