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J Virol. 1974 October; 14(4): 1008-1012
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Cold Lability of Bacillus cereus Bacteriophage CP-51

¹ Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01002

ABSTRACT

Phage CP-51 was rapidly inactivated when stored at the usual refrigerator temperatures (2 to 4 C) and even more rapidly when exposed to 0 C. The loss in viability resulting from exposure to cold appeared to correlate with the increase in number of phage particles having contracted tails. High concentrations (0.01 M) of Mg²⁺, Ca²⁺, or Mn²⁺ stabilized the phage considerably, but even in the presence of these divalent cations, it was much less stable at 0 C than at 15 C.