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J Virol. 1974 September; 14(3): 709-712
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Mutants of Bacillus subtilis Bacteriophage e Defective in dTTP-dUTP Nucleotidohydrolase

^a Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48104

ABSTRACT

Mutants of bacteriophage e inducing only 1 to 5% of wild-type levels of dTTP-dUTP nucleotidohydrolase give normal bursts of viable progeny phage whose DNA contains 5 to 10% thymine (but no uracil) in place of 5-hydroxymethyluracil. The relative heat lability of one phage mutant enzyme solubilized from the membrane fraction of infected cells suggests that a e gene codes for the induced dTTPase-dUTPase.

¹ Present address: Department of Chemistry, University of Nevada, Las Vegas, Nev. 89154.