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Properties of Feline Leukemia Virus II. In Vitro Labeling of the Polypeptides ¹

^a Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan 48824

ABSTRACT

Radioactive labeling of proteins from guanidine-hydrochloride disrupted feline leukemia virus was done in vitro by attaching [³H]methyl groups to protein amino groups with reductive alkylation. When in vitro labeled disrupted virus was analyzed by gel filtration in the presence of 6 M guanidine-hydrochloride the expected six major and one minor protein peaks were detected. When the same preparation was analyzed by immunodiffusion, the three antigens tested were found to have retained their antigenic activity.

² Present Address: School of Veterinary Medicine, University of Pennsylvania, New Bolton Center, Kennett Square R.D. 1, Pa.

¹ Article no. 6572 from the Michigan Agricultural Experiment Station. Presented at the Oncornavirus Antigen Workshop held at the Sloan-Kettering Institute, N.Y., June 1973.