JVI Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cancedda, R.
Right arrow Articles by Schlesinger, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cancedda, R.
Right arrow Articles by Schlesinger, M. J.

 Previous Article  |  Next Article 

J Virol. 1974 September; 14(3): 664-671
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Viral Proteins Formed in a Cell-Free RABBIT Reticulocyte System Programmed with RNA from a Temperature-Sensitive Mutant of Sindbis Virus

Ranieri Cancedda1, Richard Swanson and Milton J. Schlesinger

a Department of Microbiology, Division of Biology and Biomedical Sciences, Washington University, St. Louis, Missouri 63110

ABSTRACT

Viral messenger RNA was isolated from BHK cells infected with a temperature-sensitive mutant of Sindbis virus and was further purified using an oligo(dT) column. Addition of this mRNA cell-free extracts from rabbit reticulocytes led to formation of discrete authentic viral capsid protein when the reaction was performed at 29 C. However, this same protein-synthesizing system failed to make discrete viral capsid when incubated with the viral RNA at 39 C. Instead, larger-molecular-weight polypeptides that contained the viral capsid peptide sequences were produced. The inability to make a separate viral capside protein in vitro at elevated temperatures by the mRNA from this mutant exactly mimics the phenotype of this ts mutant in viral-infected cells. Three mechanisms are discussed that might account for a temperature-sensitive release of capsid. One of these is based on a model in which there are multiple sites for initiation of translation of polypeptides on a polycistronic viral mRNA.

FOOTNOTES

1 Present address: 2nd Faculty of Medicine, 2nd Institute of Biochemistry, University of Naples, Naples, Italy.

J Virol. 1974 September; 14(3): 664-671
Copyright © 1974 American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Mol. Cell. Biol. Microbiol. Mol. Biol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1974 by the American Society for Microbiology. All rights reserved.