This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Humphries, E. H. Articles by Temin, H. M. Search for Related Content PubMed PubMed Citation Articles by Humphries, E. H. Articles by Temin, H. M.

Requirement for Cell Division for Initiation of Transcription of Rous Sarcoma Virus RNA

McArdle Laboratory, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

Stationary chicken embryo fibroblasts exposed to Rous sarcoma virus (RSV) did not synthesize virus RNA until after division of the infected cells. Initiation of RNA transcription after cell division resulted in transcription of the entire viral genome and was followed shortly thereafter by appearance of progeny virus. Addition of colchicine to RSV-infected stationary chicken embryo fibroblasts resulted in inhibition of cell division and a 24-h delay in the appearance of virus RNA. RSV-infected chicken embryo fibroblasts made stationary 10 to 15 cell generations after infection continued to produce an amount of progeny virus similar to that produced in dividing RSV-infected cells. Teh virus produced from cells made stationary after virus production had begun contained RNA synthesized in stationary cells. Therefore, division of the RSV-infected chicken embryo fibroblast is required for the initiation of transcription of virus RNA, but it is not required for the maintenance of transcription of virus RNA.

This article has been cited by other articles:

• Sherman, M. P., de Noronha, C. M. C., Eckstein, L. A., Hataye, J., Mundt, P., Williams, S. A. F., Neidleman, J. A., Goldsmith, M. A., Greene, W. C. (2003). Nuclear Export of Vpr Is Required for Efficient Replication of Human Immunodeficiency Virus Type 1 in Tissue Macrophages. J. Virol. 77: 7582-7589 [Abstract] [Full Text]  
• Imamichi, T., Murphy, M. A., Adelsberger, J. W., Yang, J., Watkins, C. M., Berg, S. C., Baseler, M. W., Lempicki, R. A., Guo, J., Levin, J. G., Lane, H. C. (2002). Actinomycin D Induces High-Level Resistance to Thymidine Analogs in Replication of Human Immunodeficiency Virus Type 1 by Interfering with Host Cell Thymidine Kinase Expression. J. Virol. 77: 1011-1020 [Abstract] [Full Text]  
• Werner, S., Hindmarsh, P., Napirei, M., Vogel-Bachmayr, K., Wohrl, B. M. (2002). Subcellular Localization and Integration Activities of Rous Sarcoma Virus Reverse Transcriptase. J. Virol. 76: 6205-6212 [Abstract] [Full Text]  
• Eckstein, D. A., Sherman, M. P., Penn, M. L., Chin, P. S., De Noronha, C. M.C., Greene, W. C., Goldsmith, M. A. (2001). HIV-1 Vpr Enhances Viral Burden by Facilitating Infection of Tissue Macrophages but Not Nondividing CD4+ T Cells. J. Exp. Med. 194: 1407-1419 [Abstract] [Full Text]  
• Hatziioannou, T., Goff, S. P. (2001). Infection of Nondividing Cells by Rous Sarcoma Virus. J. Virol. 75: 9526-9531 [Abstract] [Full Text]  
• Sherman, M. P., de Noronha, C. M. C., Heusch, M. I., Greene, S., Greene, W. C. (2001). Nucleocytoplasmic Shuttling by Human Immunodeficiency Virus Type 1 Vpr. J. Virol. 75: 1522-1532 [Abstract] [Full Text]  
• Gupta, K., Ott, D., Hope, T. J., Siliciano, R. F., Boeke, J. D. (2000). A Human Nuclear Shuttling Protein That Interacts with Human Immunodeficiency Virus Type 1 Matrix Is Packaged into Virions. J. Virol. 74: 11811-11824 [Abstract] [Full Text]  
• Kootstra, N. A., Zwart, B. M., Schuitemaker, H. (2000). Diminished Human Immunodeficiency Virus Type 1 Reverse Transcription and Nuclear Transport in Primary Macrophages Arrested in Early G1 Phase of the Cell Cycle. J. Virol. 74: 1712-1717 [Abstract] [Full Text]  
• Unutmaz, D., KewalRamani, V. N., Marmon, S., Littman, D. R. (1999). Cytokine Signals Are Sufficient for HIV-1 Infection of Resting Human T Lymphocytes. J. Exp. Med. 189: 1735-1746 [Abstract] [Full Text]  
• Jenkins, Y., McEntee, M., Weis, K., Greene, W. C. (1998). Characterization of HIV-1 Vpr Nuclear Import: Analysis of Signals and Pathways. J. Cell Biol. 143: 875-885 [Abstract] [Full Text]  
• Gallay, P., Hope, T., Chin, D., Trono, D. (1997). HIV-1 infection of nondividing cells through the recognition of integrase by the importin/karyopherin pathway. Proc. Natl. Acad. Sci. USA 94: 9825-9830 [Abstract] [Full Text]  
• Dutta, A, Stoeckle, M Y, Hanafusa, H (1990). Serum and v-src increase the level of a CCAAT-binding factor required for transcription from a retroviral long terminal repeat.. Genes Dev. 4: 243-254 [Abstract]  
• Temin, H. (1976). The DNA provirus hypothesis. Science 192: 1075-1080 [Abstract]  
• Gallagher, R., Gallo, R. (1975). Type C RNA tumor virus isolated from cultured human acute myelogenous leukemia cells. Science 187: 350-353 [Abstract]