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Isolation and Characterization of Prototrophic Mutants of Escherichia coli Unable to Support the Intracellular Growth of T7

Department of Biochemistry, University of California, Berkeley, California 94720

ABSTRACT

Mutants of E. coli B/1 were isolated which grew normally but did not permit the intracellular growth of bacteriophage T7. Two classes of mutants were studied in detail (tsnB^– and tsnC^–). These strains adsorbed T7 normally and were killed by the infection. Synthesis of T7 RNA and of early and late classes of T7 proteins occurred normally after infection. In T7-infected tsnB^– cells, T7 DNA synthesis stopped prematurely shortly after its onset, suggesting that the tsnB function affects a step in the late phase of T7 DNA replication. Mutants of T7 were isolated (T7ß) which could grow on tsnB^– cells. In T7-infected tsnC^– cells, T7 DNA synthesis was completely blocked, suggesting that the tsnC function affects a step in an early phase of T7 DNA replication.

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