This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bartlett, N. M. Articles by Bellamy, A. R. Search for Related Content PubMed PubMed Citation Articles by Bartlett, N. M. Articles by Bellamy, A. R.

Electron Microscopy Study of Reovirus Reaction Cores

Department of Cell Biology, University of Auckland, Auckland, New Zealand

ABSTRACT

A combined staining and shadowing method has been used to enhance the contrast of both reovirus cores and the RNA product that they have synthesized. After limited synthesis when all RNA strands associated with the core are nascent, many cores are observed that have more than 10 strands associated with each particle. The lengths of individual nascent mRNA molecules are not identical: many strands are extruded in the form of loops. These observations are consistent with the notion that synthesis of individual mRNA molecules occurs simultaneously at different rates at a number of different sites. The sites of extrusion are probably the 12 hollow projections located on the surface of the core.

This article has been cited by other articles:

• Roy, P. (2008). Bluetongue virus: dissection of the polymerase complex. J. Gen. Virol. 89: 1789-1804 [Abstract] [Full Text]  
• Kim, J., Parker, J. S. L., Murray, K. E., Nibert, M. L. (2004). Nucleoside and RNA Triphosphatase Activities of Orthoreovirus Transcriptase Cofactor {micro}2. J. Biol. Chem. 279: 4394-4403 [Abstract] [Full Text]  
• Miller, C. L., Broering, T. J., Parker, J. S. L., Arnold, M. M., Nibert, M. L. (2003). Reovirus {sigma}NS Protein Localizes to Inclusions through an Association Requiring the {micro}NS Amino Terminus. J. Virol. 77: 4566-4576 [Abstract] [Full Text]  
• Broering, T. J., McCutcheon, A. M., Centonze, V. E., Nibert, M. L. (2000). Reovirus Nonstructural Protein {micro}NS Binds to Core Particles but Does Not Inhibit Their Transcription and Capping Activities. J. Virol. 74: 5516-5524 [Abstract] [Full Text]  
• Keroack, M, Fields, B. (1986). Viral shedding and transmission between hosts determined by reovirus L2 gene. Science 232: 1635-1638 [Abstract]