JVI Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Honess, R. W.
Right arrow Articles by Roizman, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Honess, R. W.
Right arrow Articles by Roizman, B.
J Virol. 1974 July; 14(1): 8-19
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Regulation of Herpesvirus Macromolecular Synthesis I. Cascade Regulation of the Synthesis of Three Groups of Viral Proteins 1

Robert W. Honess and Bernard Roizman

a Departments of Microbiology and Biophysics, University of Chicago, Chicago, Illinois 60637

ABSTRACT

Based on evidence that 50% of herpes simplex 1 DNA is transcribed in HEp-2 cells in the absence of protein synthesis we examined the order and rates of synthesis of viral polypeptides in infected cells after reversal of cycloheximide- or puromycin-mediated inhibition of protein synthesis. These experiments showed that viral polypeptides formed three sequentially synthesized, coordinately regulated groups designated {alpha}, ß, and {gamma}. Specifically: (i) The {alpha} group, containing one minor structural and several nonstructural polypeptides, was synthesized at highest rates from 3 to 4 h postinfection in untreated cells and at diminishing rates thereafter. The ß group, also containing minor structural and nonstructural polypeptides, was synthesized at highest rates from 5 to 7 h and at decreasing rates thereafter. The {gamma} group containing major structural polypeptides was synthesized at increasing rates until at least 12 h postinfection. (ii) The synthesis of {alpha} polypeptides did not require prior infected cell protein synthesis. In contrast, the synthesis of ß polypeptides required both prior {alpha} polypeptide synthesis as well as new RNA synthesis, since the addition of actinomycin D immediately after removal of cycloheximide precluded ß polypeptide synthesis. The function supplied by the {alpha} polypeptides was stable since interruption of protein synthesis after {alpha} polypeptide synthesis began and before ß polypeptides were made did not prevent the immediate synthesis of ß polypeptides once the drug was withdrawn. The requirement of {gamma} polypeptide synthesis for prior synthesis of ß polypeptides seemed to be similar to that of ß polypeptides for prior synthesis of the {alpha} group. (iii) The rates of synthesis of {alpha} polypeptides were highest immediately after removal of cycloheximide and declined thereafter concomitant with the initiation of ß polypeptide synthesis; this decline in {alpha} polypeptide synthesis was less rapid in the presence of actinomycin D which prevented the appearance of ß and {gamma} polypeptides. The decrease in rates of synthesis of ß polypeptides normally occurring after 7 h postinfection was also less rapid in the presence of actinomycin D than in its absence, whereas ongoing synthesis of {gamma} polypeptides at this time was rapidly reduced by actinomycin D. (iv) Inhibitors of DNA synthesis (cytosine arabinoside or hydroxyurea) did not prevent the synthesis of {alpha}, ß, or {gamma} polypeptides, but did reduce the amounts of {gamma} polypeptides made.

FOOTNOTES

1 Dedicated to the memory of Gordon Plummer.

J Virol. 1974 July; 14(1): 8-19
Copyright © 1974 American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Mol. Cell. Biol. Microbiol. Mol. Biol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1974 by the American Society for Microbiology. All rights reserved.