This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Berget, S. M. Articles by McCorquodale, D. J. Search for Related Content PubMed PubMed Citation Articles by Berget, S. M. Articles by McCorquodale, D. J.

 Previous Article  |  Next Article 

J Virol. 1974 July; 14(1): 78-85
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Isolation and Partial Characterization of Bacteriophage T5 Mutants Deficient in the Ability to Induce Deoxynucleoside Monophosphate Kinase ¹

Department of Biochemistry, University of Minnesota, St. Paul, Minnesota 55101
Department of Biology, University of Texas at Dallas, Dallas Texas 75230

ABSTRACT

Two mutants of bacteriophage T5 deficient in the ability to induce wild-type levels of deoxynucleoside monophosphate kinase were isolated and partially characterized. Both mutations were demonstrated to be in a structural gene for the kinase. One of the mutants, designated dnk 10, induces no detectable levels of dCMP, dGMP, or dTMP kinase activity. Because the mutant can successfully infect nonpermissive cells, phage-induced deoxynucleoside monophosphate kinase appears to be an unessential function for phage production. DNA synthesis in dnk 10-infected cells, however, is reduced to 30% of that observed in wild-type-infected cells; phage production is reduced by a comparable amount. The dnk mutation has been mapped and located on the "C" region of the T5 genetic map, 6.3 map units from the C1 locus.

¹ Scientific paper no. 8625 of the Minnesota Agricultural Experimental Station.