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Physical and Chemical Properties of an Oncornavirus Associated with a Murine Adrenal Carcinoma Cell Line

Departments of Microbiology and Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232

ABSTRACT

A type C oncornavirus has been isolated from a continuous cell line of murine adrenal carcinoma in culture. The particles have a buoyant density of 1.165 g/cm³, exhibit typical type C morphology by electron microscopy, possess an RNA-dependent DNA polymerase, and have a high molecular weight RNA (6.1 x 10⁶) which can be denatured to a homogeneous lower molecular weight species (3.2 x 10⁶) when extracted from rapidly harvested "immature" virions. The virus is related antigenically to other mammalian oncornaviruses and exhibits a similar, although much more complex, sodium dodecyl sulfate gel electrophoretic profile of virion proteins when compared to the profiles of other type C RNA tumor viruses.