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J Virol. 1974 June; 13(6): 1331-1337
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Origin of Reovirus Oligo(A)

^a Roche Institute of Molecular Biology, Department of Cell Biology, Nutley, New Jersey 07110

ABSTRACT

Reovirus contains about 1,200 molecules per virion of oligo(A) of chain length 10 to 15 nucleotides in addition to the 10 double-stranded genome segments. Virions purified from infected BHK, HeLa, or L cells had similar amounts of oligo(A) of the same composition, indicating that it is a virus-specific product. Although conversion of virions to cores by chymotryptic digestion resulted in an almost complete loss of oligo(A) and a marked decrease in infectivity, the infectivity could be partially restored by adsorbing cores to cells in the presence of Kaopectate. Core-infected cells yielded virions that contained a normal complement of oligo(A). The results indicate that oligo(A) is not essential for infectivity or required as a primer/template for its own synthesis.

¹ Present address: Department of Microbiology, Vanderbilt University School of Medicine, Nashville, Tenn. 37232.

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