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J Virol. 1974 June; 13(6): 1254-1262
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Base Composition and Hybridization Studies of the Three Double-Stranded RNA Segments of Bacteriophage {varphi}6 1

J. L. Van Etten, A. K. Vidaver, R. K. Koski and J. P. Burnett

Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska 68503
Lilly Research Laboratories, Indianapolis, Indiana 46206

ABSTRACT

The three double-stranded ribonucleic acid (dsRNA) segments of the bacteriophage {varphi}6 were isolated and shown to have similar melting temperatures and base compositions. RNA:RNA hybridization experiments with the isolated segments eliminate the possibility that the two smaller dsRNA segments arise from a cleavage of the large dsRNA segment. The two smaller RNA segments reanneal rapidly even at low temperatures; in contrast, the large dsRNA reannealed only at higher temperatures. Evidence is also presented which suggests that the dsRNAs may contain a short single-stranded RNA tail.

FOOTNOTES

1 Journal paper no. 3729, Nebraska Agricultural Experiment Station.

J Virol. 1974 June; 13(6): 1254-1262
Copyright © 1974 American Society for Microbiology. All Rights Reserved.



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Copyright © 1974 by the American Society for Microbiology. All rights reserved.