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Evidence for Heterogeneity in Populations of T5 Bacteriophage

¹ Laboratoire de Biochimie, Institut du Radium-Fondation Curie, Université Paris-XI, Bâtiment 110, 91405-Orsay, France

ABSTRACT

Each T5 stock contains a population of particular phages that, just after adsorption onto the host bacteria. release their entire chromosome outside the bacterial membrane in a place where it is sensitive to bacterial enzymes. This release takes place before the sensitization step to deprivation of calcium and before the transfer of the first-step DNA fragment. Secondarily, this released DNA is degraded by bacterial enzymes, mainly by the endonuclease I; the products of degradation are spontaneously released in the surrounding medium. Thus, in each T5 phage stock it seems that there is a minor population that is deficient for the mechanism of controlled DNA injection into the bacteria.

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• de Frutos, M., Letellier, L., Raspaud, E. (2005). DNA Ejection from Bacteriophage T5: Analysis of the Kinetics and Energetics. Biophys. J 88: 1364-1370 [Abstract] [Full Text]  
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