This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Neiman, P. E. Articles by MacDonnell, D. Search for Related Content PubMed PubMed Citation Articles by Neiman, P. E. Articles by MacDonnell, D.

 Previous Article  |  Next Article 

J Virol. 1974 April; 13(4): 837-846
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Nucleotide Sequence Relationships of Avian RNA Tumor Viruses: Measurement of the Deletion in a Transformation-Defective Mutant of Rous Sarcoma Virus

Department of Medicine, University of Washington School of Medicine, Seattle, Washington 98195
U.S. Public Health Service Hospital, Seattle, Washington 98114

ABSTRACT

Stocks of cloned helper-independent Rous sarcoma virus (RSV) spontaneously segregate transformation-defective (td) mutants that appear to have an RNA genome composed of smaller subunits than those of the patent virus. Differential hybridization and competitive hybridization techniques involving reactions between viral RNA and proviral sequences in host cell DNA (under conditions of initial DNA excess) were used to measure the extent of the deletion in a td mutant of Prague strain (Pr) of RSV (Pr RSV-C). Viral 60 to 70S RNA sequences labeled to 1 to 5 x 10⁷ counts per min per µg with ¹²⁵I were characterized with respect to their properties in hybridization reactions and used to reinforce data obtained with [³H]RNA of lower specific activity. By these techniques, about 13% ± 3% of the sequences Pr RSV-C that formed hybrids with DNA from virus-induced sarcomas appeared to be deleted from the genome of td Pr RSV-C. Studies comparing hybridization of RNA from Pr RSV-C and td Pr RSV-C with RSV-related sequences in normal cells, and competition experiments with RNA from the endogenous chicken oncornavirus Rous-associated virus type 0 (RAV-0) provided evidence that the majority, if not all, of the RNA sequences of Pr RSV-C deleted from its transformation-defective mutant are not represented in normal chicken DNA. Competition studies with a leukosis virus, RAV-7, indicated this virus also lacks a genome segment of about the same size as the deletion in the td mutant. Finally, the genome of all three "exogenous" viruses was found to lack a small segment (about 12%) of sequences present in the endogenous provirus of RAV-O.

¹ Present address: University of Washington, Department of Medicine, Division of Oncology, Seattle, Washington 98195.

This article has been cited by other articles:

• Callahan, R, Lieber, M., Todaro, G., Graves, D., Ferrer, J. (1976). Bovine leukemia virus genes in the DNA of leukemic cattle. Science 192: 1005-1007 [Abstract]