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Effects of L-Glutamine Deprivation on Growth of HVJ (Sendai Virus) in BHK Cells

Germfree Life Research Institute and First Department of Internal Medicine, Nagoya University School of Medicine, Schowa-ku, Nagoya, Japan

ABSTRACT

L-Glutamine requirement for viral maturation was found in BHK-HVJ cells, a cell line of baby hamster kidney cells persistently infected with HVJ (Sendai virus). Synthesis of envelope protein in BHK-HVJ cells was markedly suppressed by deprivation of L-glutamine, whereas development of nucleocapsid (S) antigen was less affected. More detailed examination of this phenomenon was carried out by using a cytolytic system. Growth of HVJ in BHK cells cultured in media deprived of various amino acids was investigated, and omission of L-glutamine from culture medium resulted in a marked inhibitory effect on the release of infectious virus and synthesis of envelope protein, although synthesis of virus-specific RNA and nucleocapsid antigen in the cells was readily detected. When L-glutamine was restored to the culture medium, infectious virus and envelope protein could be detected. L-Glutamic acid, L-aspartic acid, or L-alanine could be substituted for L-glutamine. Effects of L-glutamine deprivation on HVJ growth in several other cells were also investigated. The growth of HVJ in the cells other than BHK and FL cells was not suppressed by lack of L-glutamine. Growth of Sindbis virus in BHK cells was also markedly retarded in the absence of L-glutamine.