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Purification of Single- and Double-Length M13 Virions by Polyacrylamide Gel Electrophoresis

Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706
Department of Biophysics and Microbiology, University of Pittsburgh, Pittsburgh, Pennsylvania 15213

ABSTRACT

Complete separation of single- and double-length M13 virions from each other and from still-longer phage particles was achieved by electrophoresis of phage stocks through 2.5% polyacrylamide gels.

¹ Present address: Département de Microbiologie et d'Immunologie, Université de Montréal, Case postale 6128, Montréal Qué, Canada H3C 3J7.

² Present address: Department of Biochemical Sciences, Princeton University, Princeton, N. J. 08540.

³ Present address: Department of Bacteriology, University of California, Davis, Calif. 95616.