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J Virol. 1974 February; 13(2): 291-297
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Replication of Reticuloendotheliosis Viruses in Cell Culture: Acute Infection

Howard M. Temin and Victoria K. Kassner

McArdle Laboratory for Cancer Research, University of Wisconsin Medical Center, Madison, Wisconsin 53706

ABSTRACT

Replication of reticuloendotheliosis viruses (REV) in cultures of chicken and duck fibroblasts leads to some cell death soon after infection. This cell killing was used to develop a plaque assay for Trager duck spleen necrosis virus (TDSNV) on duck embryo fibroblasts. A normal replicative cell cycle was required for normal virus production and the development of cytopathic effects in chicken cells exposed to TDSNV. The latent period was about two days. Stationary chicken embryo fibroblasts could be infected by REV; DNA synthesis was required, but protein synthesis was not.

J Virol. 1974 February; 13(2): 291-297
Copyright © 1974 American Society for Microbiology. All Rights Reserved.



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