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Molecular Weight Determination of Sendai and Newcastle Disease Virus RNA

Département de Biologie Moléculaire, Université de Genève, Genève, Switzerland
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724

ABSTRACT

The molecular weights of Sendai and Newcastle disease virus RNA were estimated by sedimentation in sucrose gradients and by length measurements in the electron microscope under both denaturing and nondenaturing conditions. Sedimentation analyses under denaturing conditions yielded molecular weight estimates of 2.3 x 10⁶ to 2.6 x 10⁶, whereas length measurements yielded estimates of 5.2 x 10⁶ to 5.6 x 10⁶ for both denatured and nondenatured viral RNA. It would appear that the conditions of denaturation used (99% dimethyl sulfoxide at 26 C, and reaction with 1.1 M formaldehyde for 10 min at 60 C) do not equally denature parainfluenza virus RNA and other RNAs, such as cellular rRNA, 45S rRNA precursor, and R17 RNA.