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J Virol. 1974 January; 13(1): 42-45
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Enzymatic Radioiodination of the Envelope Proteins of Avian Myeloblastosis Virus 1

Robert B. Fritz

a Department of Microbiology, Division of Basic Health Sciences, Emory University, Atlanta, Georgia 30322

ABSTRACT

Purified avian myeloblastosis virus was iodinated by the lactoperoxidase method. Disruption of the labeled virions and chromatography of the viral proteins showed that radioiodine was associated only with the glycoproteins of the viral envelope. Reaction of the radiolabeled virus with antiviral antibody followed by mild detergent treatment and subsequent recovery of immune complexes showed this technique to be useful for the isolation of viral envelope antigens.


FOOTNOTES

1 Publication no. 1157 of the Division of Basic Health Sciences, Emory University, Atlanta, Ga.


J Virol. 1974 January; 13(1): 42-45
Copyright © 1974 American Society for Microbiology. All Rights Reserved.







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Copyright © 1974 by the American Society for Microbiology. All rights reserved.