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J Virol. 1974 January; 13(1): 36-41
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Analysis of Minimal Functions of Simian Virus 40 III. Evidence for "Host Cell Repair" of Oncogenicity and Infectivity of UV-Irradiated Simian Virus 40

¹ The Wistar Institute, Philadelphia, Pennsylvania 19104

ABSTRACT

The in vitro transforming capacity of simian virus 40 (SV40) for Syrian hamster cells is highly resistant to inactivation by UV light in comparison to infectivity. In the same cell system, we demonstrated a "host cell repair mechanism" sensitive to caffeine which is, to a large extent, responsible for the high resistance to UV inactivation of the transforming capacity of SV40. The survival of infectivity of UV-irradiated SV40 in CV-1 cells was also sensitive to caffeine, again indicating host cell repair. On the other hand, depression of normal cell DNA synthesis by hydroxyurea during the first 24 h postinfection only modestly reduced, and to a similar extent, the transforming capacity of UV-irradiated and nonirradiated SV40.