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Virus-Induced Cytoplasmic Membrane Structures Associated with Semliki Forest Virus Infection Studied by the Freeze-Etching Method

The Electron Microscope Laboratory and The Third Department of Pathology, University of Helsinki, Helsinki, Finland

ABSTRACT

Intracellular membrane structures associated with the Semliki Forest virus replication process were studied from freeze-etch replicas. Cleaved membrane structures inside the CPV I type vacuoles lacked the typical membrane particles present on most other fractured membranes. CPV II type vacuoles present in thin sections were obscured in the freeze-etch replicas by the cytoplasmic ground substance.