This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Reese, J. F. Articles by Bartell, P. F. Search for Related Content PubMed PubMed Citation Articles by Reese, J. F. Articles by Bartell, P. F.

 Previous Article  |  Next Article 

J Virol. 1974 January; 13(1): 22-27
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Factors Influencing the Adsorption of Bacteriophage 2 to Cells of Pseudomonas aeruginosa

¹ Department of Microbiology, College of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, New Jersey 07103

ABSTRACT

Phage 2 adsorbed to Pseudomonas aeruginosa strain BI in 5 mM Tris buffer, providing that cations like Na⁺, Mg²⁺, or Ca²⁺ were present. Adsorption was observed over a broad pH range, reaching a maximum level around pH 7.5, which coincided with the pH required for maximal activity of the phage 2-associated slime polysaccharide depolymerase. Mutants of strain BI and other strains of P. aeruginosa possessing slime layers that were devoid of phage 2 depolymerase substrate were incapable of adsorbing phage 2. On the other hand, those strains containing substrate for the phage 2 depolymerase in the slime layer were capable of adsorbing phage 2. The same relationship of phage depolymerase-substrate interaction to phage adsorption was observed with Pseudomonas phage 8, which possesses a depolymerase that differs in its specificity from the phage 2 depolymerase. The receptor-like activity of purified slime containing the specific substrate for the phage-associated depolymerase was demonstrable by its ability to inactivate phage. However, receptor-like activity or phage inactivation was not observed with those slimes that were devoid of the depolymerase substrate.

This article has been cited by other articles:

• Lagoumintzis, G., Christofidou, M., Dimitracopoulos, G., Paliogianni, F. (2003). Pseudomonas aeruginosa Slime Glycolipoprotein Is a Potent Stimulant of Tumor Necrosis Factor Alpha Gene Expression and Activation of Transcription Activators Nuclear Factor {kappa}B and Activator Protein 1 in Human Monocytes. Infect. Immun. 71: 4614-4622 [Abstract] [Full Text]