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J Virol. 1974 January; 13(1): 205-210
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Viruses of Entamoeba histolytica IV. Studies on the Nucleic Acids of the Filamentous and Polyhedral Viruses

¹ Laboratories of Viral Diseases and Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20014

ABSTRACT

The nucleic acids of two amoebal viruses were studied by several independent methods. The filamentous virus, V_ABRM, was shown to be inhibited by bromodeoxyuridine, iododeoxyuridine, and cytosine arabinoside. With acridine orange staining, V_ABRM inclusions appeared greenish-yellow, indicating that these contained double-stranded nucleic acid. The polyhedral virus, V₃₀₁, was also inhibited by bromodeoxyuridine, iododeoxyuridine, and cytosine arabinoside. In addition, nucleic acid hybridization showed that a new DNA species was synthesized in infected amoebal cultures. The intracellular localization of this new DNA was consistent with previous electron microscope studies of the cytoplasmic maturation of V₃₀₁.