This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Shimizu, K. Articles by Sekiguchi, M. Search for Related Content PubMed PubMed Citation Articles by Shimizu, K. Articles by Sekiguchi, M.

Biochemical Studies on the Mutation of Bacteriophage T4: Differential Inhibition of ⁺ and DNA Synthesis by Mitomycin C

¹ Division of Molecular Genetics, Department of Biology, Faculty of Science, Kyushu University, Fukuoka, 812, Japan

ABSTRACT

Biochemical studies were carried out to determine the effect of mutation on T4 DNA synthesis. The rate and final extent of DNA synthesis are almost the same with T4D- and T4-infected cells, although the burst size of T4 is about one-sixth that of the wild type. The DNA synthesis of T4-infected cells is more readily inhibited by mitomycin C than is that of T4 wild type. When mitomycin C was added during active phage growth, DNA synthesis of T4 halted almost immediately. T4 DNA polymerases isolated from ⁺- and -infected cells, however, exhibit no difference with regard to their sensitivities to mitomycin C, priming activities with alkylated or ultraviolet light-irradiated templates and other enzymatic properties.