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J Virol. 1973 December; 12(6): 1434-1441
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Translation of Virus mRNA: Synthesis of Bacteriophage Qß Proteins in a Cell-Free Extract from Wheat Embryo

Biophysics Laboratory and Biochemistry Department, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

The RNA from bacteriophage Qß can be translated by cell-free extracts from wheat embryos. This translation, by 80S ribosomes, occurs at a low magnesium ion concentration. Three products are synthesized which coelectrophorese with Qß proteins synthesized in Escherichia coli extracts. The smallest of these has been identified as coat protein. Although the polycistronic bacteriophage message is translated with fidelity, the efficiency is much less than when the monocistronic brome mosaic virus coat protein message is translated.