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J Virol. 1973 December; 12(6): 1253-1258
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Synthesis of Bacteriophage and Host DNA in Toluene-Treated Cells Prepared from T4-Infected Escherichia coli: Role of Bacteriophage Gene D2a

^a Department of Biology, Rice University, Houston, Texas 77001

ABSTRACT

We investigated the synthesis of DNA in toluene-treated cells prepared from Escherichia coli infected with bacteriophage T4. If the phage carry certain rII deletion mutations, those which extend into the nearby D2a region, the following results are obtained: (i) phage DNA synthesis occurs unless the phage carries certain DNA-negative mutations; and (ii) host DNA synthesis occurs even though the phage infection has already resulted in the cessation of host DNA synthesis in vivo. The latter result indicates that the phage-induced cessation of host DNA synthesis is not due to an irreversible inactivation of an essential component of the replication apparatus. If the phage are D2a⁺, host DNA synthesis in toluene-treated infected cells is markedly reduced; phage DNA synthesis is probably also reduced somewhat. These D2a effects, considered along with our earlier work, suggest that a D2a-controlled nuclease, specific for cytosine-containing DNA, is active in toluene-treated cells.

¹ Present address: Biology Department, University of Texas M.D. Anderson Hospital and Tumor Institute, Houston, Tex. 77025.

² Present address: Physiologisch-chemisches Institut der Universitat, Tubingen, Germany.

³ Present address: Zoology Department, University of Texas, Austin, Tex. 78712.

⁴ Present address: Cetus Scientific Laboratories Inc., Berkeley, Calif. 94710.