This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sawyer, R. C. Articles by Dahlberg, J. E. Search for Related Content PubMed PubMed Citation Articles by Sawyer, R. C. Articles by Dahlberg, J. E.

 Previous Article  |  Next Article 

J Virol. 1973 December; 12(6): 1226-1237
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Small RNAs of Rous Sarcoma Virus: Characterization by Two-Dimensional Polyacrylamide Gel Electrophoresis and Fingerprint Analysis

Department of Physiological Chemistry, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

Approximately 15 to 20 different species of small (4 to 7S) RNAs have been purified by two-dimensional polyacrylamide gel electrophoresis of RNA isolated from virions of Schmidt-Ruppin D strain of Rous sarcoma virus. Each species of small RNA has been isolated free of 70S RNA; nine of them, including 5S and 7S RNAs, were also found associated with the 70S genomic RNA. Most of the 4S RNAs are present at an average of less than one copy per virion. The 4S RNAs have T1 RNase (EC 2.7.7.26) fingerprints, which are very similar to those of tRNAs. One of the smallest 4S RNAs, which can act as a primer for initiation of RNA-directed DNA synthesis, is associated with the 70S RNA in 1 to 2 copies per complex, whereas an additional 6 to 8 copies of this molecule are free.

This article has been cited by other articles:

• Kovaleski, B. J., Kennedy, R., Hong, M. K., Datta, S. A., Kleiman, L., Rein, A., Musier-Forsyth, K. (2006). In Vitro Characterization of the Interaction between HIV-1 Gag and Human Lysyl-tRNA Synthetase. J. Biol. Chem. 281: 19449-19456 [Abstract] [Full Text]  
• ONAFUWA-NUGA, A. A., TELESNITSKY, A., KING, S. R. (2006). 7SL RNA, but not the 54-kd signal recognition particle protein, is an abundant component of both infectious HIV-1 and minimal virus-like particles. RNA 12: 542-546 [Abstract] [Full Text]  
• Oh, J., Chang, K. W., Hughes, S. H. (2006). Mutations in the U5 Sequences Adjacent to the Primer Binding Site Do Not Affect tRNA Cleavage by Rous Sarcoma Virus RNase H but Do Cause Aberrant Integrations In Vivo. J. Virol. 80: 451-459 [Abstract] [Full Text]  
• Onafuwa-Nuga, A. A., King, S. R., Telesnitsky, A. (2005). Nonrandom Packaging of Host RNAs in Moloney Murine Leukemia Virus. J. Virol. 79: 13528-13537 [Abstract] [Full Text]  
• Wei, M., Cen, S., Niu, M., Guo, F., Kleiman, L. (2005). Defective Replication in Human Immunodeficiency Virus Type 1 When Non-Primers Are Used for Reverse Transcription. J. Virol. 79: 9081-9087 [Abstract] [Full Text]  
• GILES, K. E., CAPUTI, M., BEEMON, K. L. (2004). Packaging and reverse transcription of snRNAs by retroviruses may generate pseudogenes. RNA 10: 299-307 [Abstract] [Full Text]  
• Cen, S., Javanbakht, H., Kim, S., Shiba, K., Craven, R., Rein, A., Ewalt, K., Schimmel, P., Musier-Forsyth, K., Kleiman, L. (2002). Retrovirus-Specific Packaging of Aminoacyl-tRNA Synthetases with Cognate Primer tRNAs. J. Virol. 76: 13111-13115 [Abstract] [Full Text]  
• Gabor, J., Cen, S., Javanbakht, H., Niu, M., Kleiman, L. (2002). Effect of Altering the tRNAConcentration in Human Immunodeficiency Virus Type 1 upon Its Annealing to Viral RNA, GagPol Incorporation, and Viral Infectivity. J. Virol. 76: 9096-9102 [Abstract] [Full Text]  
• Parker, S. D., Wall, J. S., Hunter, E. (2001). Analysis of Mason-Pfizer Monkey Virus Gag Particles by Scanning Transmission Electron Microscopy. J. Virol. 75: 9543-9548 [Abstract] [Full Text]  
• Lund, A. H., Duch, M., Pedersen, F. S. (2000). Selection of functional tRNA primers and primer binding site sequences from a retroviral combinatorial library: identification of new functional tRNA primers in murine leukemia virus replication. Nucleic Acids Res 28: 791-799 [Abstract] [Full Text]  
• Vogt, V. M., Simon, M. N. (1999). Mass Determination of Rous Sarcoma Virus Virions by Scanning Transmission Electron Microscopy. J. Virol. 73: 7050-7055 [Abstract] [Full Text]  
• Lund, A. H., Schmidt, J., Luz, A., Sørensen, A. B., Duch, M., Pedersen, F. S. (1999). Replication and Pathogenicity of Primer Binding Site Mutants of SL3-3 Murine Leukemia Viruses. J. Virol. 73: 6117-6122 [Abstract] [Full Text]