This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Shoyab, M. Articles by Baluda, M. A. Search for Related Content PubMed PubMed Citation Articles by Shoyab, M. Articles by Baluda, M. A.

 Previous Article  |  Next Article 

J Virol. 1973 September; 12(3): 534-537
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Separation of DNA Sequences Complementary to the RNA of Avian Myeloblastosis Virus from Chicken DNA by Alkaline Cesium Chloride Density Sedimentation

Department of Microbiology and Immunology, University of California, Los Angeles, California 90024

ABSTRACT

Density gradient sedimentation in alkaline cesium chloride of DNA from normal chicken embryos or leukemic myeloblasts fragmented to a size of 13S revealed that the DNA sequences complementary to 70S avian myeloblastosis virus RNA sedimented in the high guanine plus cytosine region ahead of the main peak of cellular DNA. When the DNA was fragmented into pieces of 6.6S there was a broader distribution of the DNA sequences complementary to the viral RNA. This technique could be employed as a step towards the isolation of DNA copies of the entire viral RNA genome from the mass of host cellular DNA.