This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Falcoff, E. Articles by Revel, M. Search for Related Content PubMed PubMed Citation Articles by Falcoff, E. Articles by Revel, M.

Next Article 

J Virol. 1973 September; 12(3): 421-430
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Correlation Between the Antiviral Effect of Interferon Treatment and the Inhibition of In Vitro mRNA Translation in Noninfected L cells

Institut du Radium, Paris, France
Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel

ABSTRACT

When noninfected L-cell suspension cultures are treated with interferon (specific activities superior to 10⁶ reference units per mg of protein), the cell-free cytoplasmic extracts obtained are inactive for the translation of exogenous natural mRNAs. The dose-response curve shows that comparable amounts of interferon are required to produce a 50% reduction of Mengo virus multiplication in vivo and Mengo RNA translation in vitro. With higher doses of interferon, Mengo RNA translation is completely abolished, while poly U translation and endogenous protein synthesis are only slightly affected. The inactivation of Mengo RNA translation is reversible; after removal of interferon, normal translation activity is regained together with the ability to support Mengo virus multiplication. Fractionation of the cell-free extracts shows that the effect is localized in the fraction which can be washed off the ribosomes by high salt. These results establish that interferon induces a block in genetic translation in noninfected L cells.

This article has been cited by other articles:

• Lekmine, F., Uddin, S., Sassano, A., Parmar, S., Brachmann, S. M., Majchrzak, B., Sonenberg, N., Hay, N., Fish, E. N., Platanias, L. C. (2003). Activation of the p70 S6 Kinase and Phosphorylation of the 4E-BP1 Repressor of mRNA Translation by Type I Interferons. J. Biol. Chem. 278: 27772-27780 [Abstract] [Full Text]  
• De Maeyer-Guignard, J, Cachard, A, De Maeyer, E (1975). Delayed-type hypersensitivity to sheep red blood cells: inhibition of sensitization by interferon. Science 190: 574-576 [Abstract]