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J Virol. 1973 August; 12(2): 360-366
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Effect of Viral Double-Stranded RNA on Mammalian Cells in Culture: Cytotoxicity Under Conditions Preventing Viral Replication and Protein Synthesis

^a Department of Microbiology, Indiana University, Bloomington, Indiana 47401

ABSTRACT

Noninfectious bovine enterovirus double-stranded RNA induces cytopathic effects when added to mammalian cells in culture. This is demonstrated by ⁵¹Cr release from prelabeled murine lymphoma cells and trypan blue uptake. Also, the induction of cell death by this viral RNA occurs in the presence of inhibitors of protein synthesis (cycloheximide and puromycin). The possible role and mechanism of viral, double-stranded RNA as a cytopathic agent in virally infected cells are discussed.

¹ Current address: Department of Microbiology, University of California Medical Center, San Francisco, Calif. 94122.