This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Shah, D. B. Articles by Berger, H. Search for Related Content PubMed PubMed Citation Articles by Shah, D. B. Articles by Berger, H.

 Previous Article  |  Next Article 

J Virol. 1973 August; 12(2): 328-333
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Effect of a Gene-Specific Suppressor Mutation (das) on DNA Synthesis of Gene 46-47 Mutants of Bacteriophage T4D ¹

^a Department of Cell Biology, University of Kentucky Medical School, Lexington, Kentucky 40506
Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218

ABSTRACT

Mutants in genes 46 and 47 of bacteriophage T4 exhibit early cessation of DNA synthesis, inability to form a normal rapidly sedimenting DNA intermediate (200S), reduced genetic recombination, and reduced viable phage production. A gene-specific suppressor mutation called das partially restores many of the pleiotropic effects of gene 46-47 mutants (13). Our results indicate that this partial suppression by das is associated with (i) the synthesis of a small fraction of DNA containing long single chains not detectable in 46-47 infection and (ii) a decrease in an "early" function which participates in the degradation of DNA synthesized in the absence of 46-47 functions. However, das does not restore the formation of a normal rapidly sedimenting (200S) DNA intermediate.

¹ This paper constitutes contribution no. 727 of the Department of Biology, The Johns Hopkins University.