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J Virol. 1973 July; 12(1): 99-107
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Relationship Between Replication of Simian Virus 40 DNA and Specific Events of the Host Cell Cycle

Unité de Physiologie des Virus, Institut de Recherches Scientifiques sur le Cancer, B.P. n° 8, 94800 Villejuif, France

ABSTRACT

The relationship between replication of simian virus 40 (SV40) DNA and the various periods of the host-cell cycle was investigated in synchronized CV₁ cells. Cells synchronized through a double excess thymidine procedure were infected with SV40 at the beginning or the middle of S, or in G₂. The first viral progeny DNA molecules were in all instances detected approximately 20 h after release from the thymidine block, independent of the time of infection. The length of the early, prereplicative phase of the virus growth cycle therefore depended upon the period of the cell cycle at which the cells were infected. Infection with SV40 was also performed on cells obtained in early G₁ through selective detachment of cells in metaphase. As long as the cells were in G₁ at the time of infection, the first viral progeny DNA molecules were detected during the S period immediately following, whereas if infection took place once the cells had entered S, no progeny DNA molecule could be detected until the S period of the next cell cycle. These results suggest that the infected cell has to pass through a critical stage situated in late G₁ or early S before SV40 DNA replication can eventually be initiated.

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