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J Virol. 1973 July; 12(1): 33-38
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Effects of Actinomycin D and Ultraviolet and Ionizing Radiation on Pichinde Virus

Michael F. Carter, Frederick A. Murphy, J. Pierre Brunschwig, Christine Noonan and William E. Rawls

Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas 77025
National Communicable Disease Center, Atlanta, Georgia 30333

ABSTRACT

Actinomycin D (0.05 µg/ml) suppresses the synthesis of ribosomal RNA of baby hamster kidney (BHK21) cells. The production of infectious Pichinde virus was enhanced in the presence of actinomycin D, although the production of virus particles was not substantially different from cultures inoculated in the absence of the drug. By prelabeling BHK21 cells with 3H-uridine and then allowing the virus to replicate in the presence of actinomycin D, it was possible to show that ribosomal RNA synthesized prior to infection was incorporated into the virion. A single-hit kinetics of inactivation of Pichinde virus was observed with ultraviolet light, suggesting that the virus contains only a single copy of genome per virion. Comparison of the inactivation kinetics by gamma irradiation of Pichinde virus with Sindbis and rubella virus indicated that the radiosensitive genome of Pichinde virus was about 6 x 106 to 8 x 106 daltons. This value is greater than the 3.2 x 106 daltons which was estimated by biochemical analysis. One possible explanation considered is that the ribosomal RNA of host cell origin is functional and accounts for the differences in genome size estimated by the two methods.

J Virol. 1973 July; 12(1): 33-38
Copyright © 1973 American Society for Microbiology. All Rights Reserved.





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Copyright © 1973 by the American Society for Microbiology. All rights reserved.